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MitoScore

The revolutionary test MitoScore

The MitoScore test is a biomapper that provides information about the energy status of the embryo. Through it, embryos are determined with the greatest potential for implantation, specifically developing pregnancy and birth. This method describes the number of copies of the mitochondrial DNA in the cytoplasm of the cells of the developing embryo.

It is well known that the genetically normal embyos are not implanted due the hich activity of the mitochondria. The published results from the researches confirm this hypothesis, that claims that the number of the mitochondrial DNA copies in the embryo is not a direct indicator for its energy capacity, but is an index for its energy stress, which can be used for implantational potencial prognosis of the embryo (Dіеz-Јuаn А. еt аl. 2015). The increased mitochondrial activity provokes more mutations in the mitochondrial DNA, increases the levels of the free radicals in the cells, which causes low levels of implantation of the embyos. With the MitoScore method, a limit of the mitochondrial activity is set, at which the embryos with increased activity are with minimal or no chance for implantation in the uterus cavity.

Mitochondria and mitochondrial DNA

Mitochondria are universal cellular organisms that are present in almost all eukaryotic cells. Sometimes mitochondria are described as “cellular energy factories”, because they produce the greater part of ATP (adenosinediphosphate), which is the source of the chemical energy for the cell. They are the energy system of the embryo. Mitochondria have their own DNA, known as mitochondrial DNA. The amount of mitochondrial DNA in the embryo is an indexation for energetic stress, which can be used for determination of its implantational potential. The studies show that the increased mitochondrial DNA in the embryo is criteria for low energy levels and low implantation potential.

What is the use of MitoScore?

This method will help specialists to choose an embryo with higher potential for implantation and consequently to increase the chances of implantation;

MitoScore provides additional information on the implantation potential of the embryonic embryos, together with the morphological assessment.

The information from the test makes it possible to identify embryos with potential in many phases: 3rd or 5th day of embryonic development;

The MitoCcop method can be used in conjunction with PGЅ / PGD, as from one and the same test, both studies can be performed. Embryos do not need to be subjected to additional interventions separately from those related to routine chromosome screening.

What are the advantages of the MitoScore method?

  • Increases the percentage of implantation and pregnancy during in vitro treatment procedures;
  • Decreasing the risk from multiple pregnancy
  • Application of additional methods for research in the early stage of embryonic development: on the 3rd or 5th day;
  • Additional information regarding the “health” of the embryo, with additional morphological observations.
  • One of the biggest advantages of the MitoScore method is that it supports the selection of embryos with the greatest potential for implantation. Studies show that the percentage of realized pregnancies can increase up to 70-80% in the case of a transfer, even of one embryo. Respectively this leads to the elimination of the risk from multiple pregnancies.

When should the MitoScore method be applied: when the embryo is early dividing (3rd day) or embryo is in the blastocyst phase (5th day)?

The answer to this question is determined by the number of cells, which can be biopsied, the quality, the risk levels for mosaicism in the embryo and last but not least – the experience of the embryologist.

Is it possible to diagnose mitochondrial diseases with MitoScore?

The answer is No. The method only gives the relative amount of mitochondrial DNA, which is an indicator of the energy content of the developing embryos.

What is the objectivity of MitoScore for the prognosis of the implantation potential of the embryo?

The high levels of mitochondrial DNA copies in the genetically normal embryo are an indicator of lower embryonic viability and lower implantation potential.Through the MitoScore method, a threshold has been established, above which embryos with increased mitochondrial activity have minimal or no chance of implantation in the uterine cavity.

What should I do, if all my embryos are with high MitoScore?

The high values of the test are an indicator for low implantation potential embryos. This does not mean that no embryo will be able to implant. In these cases, it is recommended to make an embryo transfer of the embryo or the embryos with the lowest MitoScore and the highest morphological evaluation.It is also possible to freeze the embryos in order to make an additional analysis and to develop a strategy for personalized embryo transfer and implantation. From the other side, according to the theory “for the treatment of embryos”, the procedure for freezing and unfreezing the embryos may have a repressive effect on the mitochondrial activity.

Use of MitoScore in Bulgaria:

For the first time in Bulgaria, the evolutionary method MitoScore is applied in the Specialized Hospital for Obstetrics, Gynecology and Pediatric Medicine “Sofia”.